Ethylenediamine pretreatment of corn stover facilitates high gravity fermentation with low enzyme loading

Bioresour Technol. 2018 Nov:267:227-234. doi: 10.1016/j.biortech.2018.07.030. Epub 2018 Jul 7.

Abstract

This work investigated the effect of ethylenediamine pretreatment on reducing enzyme loading in high gravity fermentation. At optimal conditions of ethylenediamine pretreatment, 85.5% lignin was removed. Enzyme adsorption analysis using a fluorescent cellulose-binding protein showed 35.2% increase of productive adsorption of enzymes to ethylenediamine pretreated biomass, which was caused by high delignification and dramatically increased surface roughness and porosity. In SScF at 15% glucan loading, up to 82.2 g/L ethanol was achieved with a relatively low enzyme loading of 3.6 FPU/g dry matter. It suggested that the remarkably high digestibility of EDA pretreated corn stover could effectively reduce the enzyme loading in the high gravity fermentation of cellulosic ethanol.

Keywords: Ethylenediamine pretreatment; High gravity fermentation; High lignin removal; Increased surface roughness and porosity; Low enzyme loading.

MeSH terms

  • Ethanol
  • Ethylenediamines / chemistry*
  • Fermentation*
  • Hydrolysis
  • Hypergravity
  • Lignin
  • Zea mays*

Substances

  • Ethylenediamines
  • Ethanol
  • Lignin