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Nature. 2018 Aug;560(7716):112-116. doi: 10.1038/s41586-018-0324-7. Epub 2018 Jul 18.

53BP1-RIF1-shieldin counteracts DSB resection through CST- and Polα-dependent fill-in.

Author information

1
Laboratory for Cell Biology and Genetics, Rockefeller University, New York, NY, USA.
2
Department of Medical and Health Sciences, Linköping University, Linköping, Sweden.
3
Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Brighton, UK.
4
Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Canada.
5
Laboratory for Cell Biology and Genetics, Rockefeller University, New York, NY, USA. delange@rockefeller.edu.

Abstract

In DNA repair, the resection of double-strand breaks dictates the choice between homology-directed repair-which requires a 3' overhang-and classical non-homologous end joining, which can join unresected ends1,2. BRCA1-mutant cancers show minimal resection of double-strand breaks, which renders them deficient in homology-directed repair and sensitive to inhibitors of poly(ADP-ribose) polymerase 1 (PARP1)3-8. When BRCA1 is absent, the resection of double-strand breaks is thought to be prevented by 53BP1, RIF1 and the REV7-SHLD1-SHLD2-SHLD3 (shieldin) complex, and loss of these factors diminishes sensitivity to PARP1 inhibitors4,6-9. Here we address the mechanism by which 53BP1-RIF1-shieldin regulates the generation of recombinogenic 3' overhangs. We report that CTC1-STN1-TEN1 (CST)10, a complex similar to replication protein A that functions as an accessory factor of polymerase-α (Polα)-primase11, is a downstream effector in the 53BP1 pathway. CST interacts with shieldin and localizes with Polα to sites of DNA damage in a 53BP1- and shieldin-dependent manner. As with loss of 53BP1, RIF1 or shieldin, the depletion of CST leads to increased resection. In BRCA1-deficient cells, CST blocks RAD51 loading and promotes the efficacy of PARP1 inhibitors. In addition, Polα inhibition diminishes the effect of PARP1 inhibitors. These data suggest that CST-Polα-mediated fill-in helps to control the repair of double-strand breaks by 53BP1, RIF1 and shieldin.

PMID:
30022158
PMCID:
PMC6072559
DOI:
10.1038/s41586-018-0324-7
[Indexed for MEDLINE]
Free PMC Article

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