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Biomed Res Int. 2018 Jun 19;2018:6242173. doi: 10.1155/2018/6242173. eCollection 2018.

Characteristic of HPV Integration in the Genome and Transcriptome of Cervical Cancer Tissues.

Li W1,2,3, Qi Y4, Cui X1, Huo Q4, Zhu L1,3, Zhang A4, Tan M5, Hong Q5, Yang Y1,3, Zhang H1,2, Liu C1,2, Kong Q1,2, Geng J1, Tian Y1,2, Kong F1,3, Man D1,3.

Author information

1
Jining Medical University, Jining, Shandong 272067, China.
2
Collaborative Innovation Center for Birth Defect Research and Transformation of Shandong Province, Jining Medical University, Jining, Shandong 272067, China.
3
Affiliated Hospital of Jining Medical University, Jining, Shandong 272067, China.
4
BGI-Shenzhen, Shenzhen 518083, China.
5
University of Chinese Academy of Sciences, 19A Yuquan Road, Shijingshan District, Beijing 100049, China.

Abstract

High-risk HPV is clearly associated with cervical cancer. HPV integration has been confirmed to promote carcinogenesis in the previous studies. In our study, a total of 285 DNA breakpoints and 287 RNA breakpoints were collected. We analyzed the characteristic of HPV integration in the DNA and RNA samples. The results revealed that the patterns of HPV integration in RNA and DNA samples differ significantly. FHIT, KLF5, and LINC00392 were the hotspot genes integrated by HPV in the DNA samples. RAD51B, CASC8, CASC21, ERBB2, TP63, TEX41, RAP2B, and MYC were the hotspot genes integrated by HPV in RNA samples. Breakpoints of DNA samples were significantly prone to the region of INTRON (P < 0.01, Chi-squared test), whereas in the RNA samples, the breakpoints were prone to EXON. Pathway analysis had revealed that the breakpoints of RNA samples were enriched in the pathways of transcriptional misregulation in cancer, cancer pathway, and pathway of adherens junction. Breakpoints of DNA samples were enriched in the pathway of cholinergic synapse. In summary, our data helped to gain insights into the HPV integration sites in DNA and RNA samples of cervical cancer. It had provided theoretical basis for understanding the mechanism of tumorigenesis from the perspective of HPV integration in the HPV-associated cervical cancers.

PMID:
30018982
PMCID:
PMC6029443
DOI:
10.1155/2018/6242173
[Indexed for MEDLINE]
Free PMC Article

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