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Anal Chim Acta. 2018 May 2;1006:83-89. doi: 10.1016/j.aca.2017.12.014. Epub 2017 Dec 28.

L-tyrosine methyl ester-stabilized carbon dots as fluorescent probes for the assays of biothiols.

Author information

1
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China; University of Science and Technology of China, Hefei, Anhui, 230029, China.
2
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China.
3
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China. Electronic address: lijingce@ciac.ac.cn.
4
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China; Department of Chemistry, Physics and Applied Mathematics, State University of New York at Stony Brook, Stony Brook, New York, 11794-3400, USA; College of Physics, Jilin University, Changchun, Jilin, 130012, China. Electronic address: jin.wang.1@stonybrook.edu.

Abstract

Over the past few decades, assays of biothiols had attracted much attention due to the essential role they played in human physiology, especially using the fluorescent analysis. In most cases, competitive mechanism was often employed, where the metal ions were often introduced as the quenchers and thiols competed with metal ions due to the high binding affinity and strong thiophilicity for 'signal-on' assays. To develop a metal ions-free approach for the assays of thiols, here, L-tyrosine methyl ester capped carbon dots (Tyr-CDs) were employed and prepared as the fluorescent probes. The as-prepared Tyr-CDs displayed narrow size distribution and distinct blue fluorescence with high quantum yield (12.9%) compared with the unmodified CDs. Moreover, Tyr-CDs exhibited higher quenching efficiency due to the efficient energy transfer between Tyr-CDs and the quinone products in the presence of tyrosinase. When the targeted biothiols was present, the catalytic reaction of the tyrosinase to the formation of quinone was inhibited and the fluorescence signal was recovered in a biothiols-concentration-dependent manner, which provided the basis for the analysis of biothiols. The practical application of the present system was demonstrated by testing the biothiols in human plasma samples and good recovery was obtained, indicating that the sensing platform we proposed hold great promise in the accurate detection of biothiols in complex biosystems.

KEYWORDS:

Biothiols analysis; Fluorescence; Human plasma; Metal ions-free; Tyr-CDs

PMID:
30016267
DOI:
10.1016/j.aca.2017.12.014
[Indexed for MEDLINE]

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