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Bioconjug Chem. 2018 Sep 19;29(9):3010-3015. doi: 10.1021/acs.bioconjchem.8b00415. Epub 2018 Aug 20.

Self-Reporting Chemically Induced Protein Proximity System Based on a Malachite Green Derivative and the L5** Fluorogen Activating Protein.

Author information

1
Department of Chemistry and Chemical Biology , University of New Mexico , 300 Terrace Street NE , Albuquerque , New Mexico 87131 , United States.
2
Department of Chemistry, Department of Biological Sciences, and Molecular Biosensor and Imaging Center , Carnegie Mellon University , Pittsburgh , Pennsylvania 15213 , United States.

Abstract

A unique chemically induced proximity method is engineered based on mutant antibody VL domain using a fluorogenic malachite green derivative as the inducer, which gives fluorescent signals upon VL domain dimerization while simultaneously inducing downstream biological effects.

PMID:
30016083
PMCID:
PMC6233874
[Available on 2019-03-19]
DOI:
10.1021/acs.bioconjchem.8b00415

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