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Ann Neurol. 2018 Aug;84(2):289-301. doi: 10.1002/ana.25288. Epub 2018 Aug 21.

Subcellular origin of mitochondrial DNA deletions in human skeletal muscle.

Author information

1
Wellcome Centre for Mitochondrial Research and Newcastle Centre for Ageing and Vitality, Institute of Neuroscience, Newcastle University, Newcastle upon Tyne, United Kingdom.
2
Institute of Neurogenetics, University of Lübeck, Lübeck, Germany.
3
Molecular and Functional Neurobiology Group, Luxembourg Center for Systems Biomedicine, University of Luxembourg, Esch-sur-Alzette, Luxembourg.
4
Department of Biochemistry, Boston University School of Medicine, Boston, MA.
5
Electron Microscopy Research Services, Newcastle University, Newcastle upon Tyne, United Kingdom.
6
Meakins-Christie Laboratories, Department of Medicine, McGill University Health Centre, Montreal, Quebec, Canada.
7
National Institute for Health Research Newcastle Biomedical Research Centre, Newcastle upon Tyne Hospitals National Health Service Foundation Trust and Newcastle University, Newcastle upon Tyne, United Kingdom.
8
Medical Research Council Lifecourse Epidemiology Unit, University of Southampton, Southampton, United Kingdom.
9
Institute of Cellular Medicine, Newcastle University, Newcastle Upon Tyne, United Kingdom.
10
Department of Psychiatry, Division of Behavioral Medicine, Columbia University Medical Center, New York, NY.
11
Department of Neurology and Columbia Translational Neuroscience Initiative, H. Houston Merritt Center, Columbia University Medical Center, New York, NY.
12
Columbia University Aging Center, Columbia University, New York, NY.

Abstract

OBJECTIVE:

In patients with mitochondrial DNA (mtDNA) maintenance disorders and with aging, mtDNA deletions sporadically form and clonally expand within individual muscle fibers, causing respiratory chain deficiency. This study aimed to identify the sub-cellular origin and potential mechanisms underlying this process.

METHODS:

Serial skeletal muscle cryosections from patients with multiple mtDNA deletions were subjected to subcellular immunofluorescent, histochemical, and genetic analysis.

RESULTS:

We report respiratory chain-deficient perinuclear foci containing mtDNA deletions, which show local elevations of both mitochondrial mass and mtDNA copy number. These subcellular foci of respiratory chain deficiency are associated with a local increase in mitochondrial biogenesis and unfolded protein response signaling pathways. We also find that the commonly reported segmental pattern of mitochondrial deficiency is consistent with the three-dimensional organization of the human skeletal muscle mitochondrial network.

INTERPRETATION:

We propose that mtDNA deletions first exceed the biochemical threshold causing biochemical deficiency in focal regions adjacent to the myonuclei, and induce mitochondrial biogenesis before spreading across the muscle fiber. These subcellular resolution data provide new insights into the possible origin of mitochondrial respiratory chain deficiency in mitochondrial myopathy. Ann Neurol 2018;84:289-301.

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