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Indian J Microbiol. 2018 Sep;58(3):345-352. doi: 10.1007/s12088-018-0728-y. Epub 2018 Apr 27.

SIV-Specific Antibodies are Elicited by a Recombinant Fowlpox Virus Co-expressing SIV Gag and envT.

Zhu Y#1,2, Du S#2, Zhang Y3, Liu J3, Guo Y1, Liu C2, Bai J2, Wang M2, Zhao F2, Cao T2, Xu W2, Bai B1,2, Zhang K2, Ma Y1, Li C4,2, Jin N1,4,2.

Author information

1
1Changchun University of Chinese Medicine, Changchun, 130117 People's Republic of China.
2
3Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, 130122 People's Republic of China.
3
4Department of Neurosurgery, First Hospital, Jilin University, Changchun, 130021 People's Republic of China.
4
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009 People's Republic of China.
#
Contributed equally

Abstract

Given the failures of past HIV-1 vaccine clinical trials, potential HIV-1 vaccine candidates should be rigorously screened in preclinical models including simian immunodeficiency virus (SIV) primate models and small animal models. In this study, we tested the immunogenicity of a recombinant fowlpox virus (rFPV) expressing the SIV gag and SIV envT (rFPVsg-se) proteins in BALB/c mice, to establish a foundation for further development. rFPVsg-se was constructed through homologous recombination techniques and purified through plaque screening assays using enhanced green fluorescent protein as the reporter gene. The integration, transcription, and translation of the SIV genes were measured by PCR (genomic DNA), RT-PCR (RNA), Western-blot, respectively. The levels of SIV-specific antibodies were assessed by ELISA following a single immunization (n = 18/group) or a prime-boost strategy (n = 24/group) with rFPVsg-se and compared to FPV and PBS controls. Residual virus was measured in distant organs following immunization using PCR. SIV-specific IgG titers against gag and gp120 were detected following single vaccination and the prime-boost. As expected the titers were higher following the prime-boost approach. The levels of Gag- and gp120-specific antibodies were significantly higher than controls (p < 0.01) 14 days after the booster immunization. Residual rFPVSg-Se was detected in the muscle at the site of injection, but not in distant organs, from day 1-7 post immunization. In summary, rFPVsg-se induced high levels of SIV-specific antibodies suggesting it may be a viable candidate for further development.

KEYWORDS:

Recombinant fowlpox virus; Residual virus; SIV gag–envT; SIV-specific antibodies

PMID:
30013279
PMCID:
PMC6023820
[Available on 2019-09-01]
DOI:
10.1007/s12088-018-0728-y

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