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Foods. 2018 Jul 16;7(7). pii: E114. doi: 10.3390/foods7070114.

Evaluation of Commercial Prototype Bacteriophage Intervention Designed for Reducing O157 and Non-O157 Shiga-Toxigenic Escherichia coli (STEC) on Beef Cattle Hide.

Author information

1
Department of Animal Science, Texas A&M University, College Station, TX 77843-2471, USA. ttolen84@tamu.edu.
2
Department of Animal Science, Texas A&M University, College Station, TX 77843-2471, USA. seasonxie@tamu.edu.
3
Department of Animal Science, Texas A&M University, College Station, TX 77843-2471, USA. tbhairgrove@tamu.edu.
4
Department of Animal Science, Texas A&M University, College Station, TX 77843-2471, USA. jason.gill@tamu.edu.
5
Department of Animal Science, Texas A&M University, College Station, TX 77843-2471, USA. matt_taylor@tamu.edu.

Abstract

Microbiological safety of beef products can be protected by application of antimicrobial interventions throughout the beef chain. This study evaluated a commercial prototype antimicrobial intervention comprised of lytic bacteriophages formulated to reduce O157 and non-O157 Shiga-toxigenic Escherichia coli (STEC) on beef cattle hide pieces, simulating commercial pre-harvest hide decontamination. STEC reduction in vitro by individual and cocktailed phages was determined by efficiency of plating (EOP). Following STEC inoculation onto hide pieces, the phage intervention was applied and hide pieces were analyzed to quantify reductions in STEC counts. Phage intervention treatment resulted in 0.4 to 0.7 log10 CFU/cm² (p < 0.01) E. coli O157, O121, and O103 reduction. Conversely, E. coli O111 and O45 did not show any significant reduction after application of bacteriophage intervention (p > 0.05). Multiplicity of infection (MOI) evaluation indicated E. coli O157 and O121 isolates required the fewest numbers of phages per host cell to produce host lysis. STEC-attacking phages may be applied to assist in preventing STEC transmission to beef products.

KEYWORDS:

Shiga-toxigenic E. coli; bacteriophages; beef safety; biocontrol; cattle hides; pre-harvest

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