Format

Send to

Choose Destination
Can J Physiol Pharmacol. 2018 Nov;96(11):1161-1170. doi: 10.1139/cjpp-2017-0730. Epub 2018 Jul 16.

The gastroprotective effect of N-acetylcysteine and genistein in indomethacin-induced gastric injury in rats.

Author information

1
a Physiology Department, Faculty of Medicine, Tanta University, Tanta, Egypt.
2
b Biochemistry Department, Faculty of Medicine, Tanta University, Tanta, Egypt.
3
c Histology Department, Faculty of Medicine, Tanta University, Tanta, Egypt.

Abstract

The protective effect of N-acetylcysteine (NAC) and genistein (GEN) on an experimental model of indomethacin (IND)-induced gastric injury was investigated. A total of 50 male rats were divided into 5 groups: (1) control, (2) IND, (3) NAC pretreated, (4) GEN pretreated, and (5) NAC+GEN pretreated. Rats in groups 3-5 were orally administered NAC (500 mg/kg), GEN (10 mg/kg), or both, respectively, once daily for 7 days before the induction of gastric injury by IND (50 mg/kg). The stomach was removed for biochemical analysis and histopathological examination. Pretreatment with NAC, GEN, or both significantly improved ulcer indices and increased nitric oxide level and superoxide dismutase activity. They also significantly decreased malondialdehyde, tumour necrosis factor α levels, and myeloperoxidase activity, and downregulated matrix metalloproteinase 9 (MMP-9) gene expression compared to the IND group. NAC alone ameliorated IND-induced apoptosis, whereas GEN only significantly increased prostaglandin E2 level. Further, coadministration of both resulted in a significantly better gastroprotective effect versus solo administration. Coadministration of NAC and GEN has an additive gastroprotective effect in IND-induced gastric injury, which may be through interaction of their potential cytoprotective, antioxidant, anti-inflammatory, and antiapoptotic mechanisms together with regulation of MMP-9 expression.

KEYWORDS:

N-acetylcysteine; N-acétylcystéine; gastroprotection; gastroprotective; genistein; génistéine; indomethacin; indométacine; matrix metalloproteinase-9; métalloprotéinase matricielle 9

PMID:
30011378
DOI:
10.1139/cjpp-2017-0730
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center