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Nucleic Acids Res. 2018 Oct 12;46(18):9338-9352. doi: 10.1093/nar/gky624.

MouR controls the expression of the Listeria monocytogenes Agr system and mediates virulence.

Author information

1
Group of Molecular Microbiology, IBMC - Institute for Molecular and Cell Biology; i3S - Institute for Research and Innovation in Health, Porto 4200-135, Portugal.
2
ICBAS- Instituto de Ciências Biomédicas Abel Salazar, University of Porto, Porto 4200-135, Portugal.
3
Group of Fish Immunology & Vaccinology, IBMC - Institute for Molecular and Cell Biology; i3S - Institute for Research and Innovation in Health, Porto 4200-135, Portugal.
4
SDV - UFR Sciences Du Vivant: Université Paris Diderot-Paris 7, Paris 75013, France.
5
Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Helsinki 00014, Finland.
6
Group of Structural Biochemistry, IBMC - Institute for Molecular and Cell Biology; i3S - Institute for Research and Innovation in Health, Porto 4200-135, Portugal.

Abstract

The foodborne pathogen Listeria monocytogenes (Lm) causes invasive infection in susceptible animals and humans. To survive and proliferate within hosts, this facultative intracellular pathogen tightly coordinates the expression of a complex regulatory network that controls the expression of virulence factors. Here, we identified and characterized MouR, a novel virulence regulator of Lm. Through RNA-seq transcriptomic analysis, we determined the MouR regulon and demonstrated how MouR positively controls the expression of the Agr quorum sensing system (agrBDCA) of Lm. The MouR three-dimensional structure revealed a dimeric DNA-binding transcription factor belonging to the VanR class of the GntR superfamily of regulatory proteins. We also showed that by directly binding to the agr promoter region, MouR ultimately modulates chitinase activity and biofilm formation. Importantly, we demonstrated by in vitro cell invasion assays and in vivo mice infections the role of MouR in Lm virulence.

PMID:
30011022
PMCID:
PMC6182135
DOI:
10.1093/nar/gky624
[Indexed for MEDLINE]
Free PMC Article

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