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J Vis Exp. 2018 Jun 28;(136). doi: 10.3791/57672.

Murine Oropharyngeal Aspiration Model of Ventilator-associated and Hospital-acquired Bacterial Pneumonia.

Author information

1
Department of Molecular Microbiology and Immunology, University of Southern California; travis.nielsen@gmail.com.
2
Department of Molecular Microbiology and Immunology, University of Southern California.
3
Department of Molecular Microbiology and Immunology, University of Southern California; Division of Infectious Diseases, Department of Medicine, LAC+USC Medical Center.

Abstract

Murine infection models are critical for understanding disease pathogenesis and testing the efficacy of novel therapeutics designed to combat causative pathogens. Infectious pneumonia is among the most common infections presented by patients in the clinic and thus warrants an appropriate in vivo model. Typical pneumonia models use intranasal inoculation, which deposits excessive organisms outside the lung, causing off-target complications and symptoms, such as sinusitis, gastritis, enteritis, physical trauma, or microparticle misting to mimic aerosol spread more typical of viral, tuberculous, or fungal pneumonia. These models do not accurately reflect the pathogenesis of typical community- or healthcare-acquired bacterial pneumonia. In contrast, this murine model of oropharyngeal aspiration pneumonia mimics the droplet route in healthcare-acquired pneumonia. Inoculating 50 µL of the bacteria suspension into the oropharynx of anesthetized mice causes reflexive aspiration, which results in pneumonia. With this model, one can examine the pathogenesis of pneumonia-causing pathogens and new treatments to combat these diseases.

PMID:
30010650
PMCID:
PMC6102004
DOI:
10.3791/57672
[Indexed for MEDLINE]
Free PMC Article

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