Format

Send to

Choose Destination
J Ethnopharmacol. 2018 Oct 28;225:336-341. doi: 10.1016/j.jep.2018.07.014. Epub 2018 Jul 31.

Neuroprotective effect of Asparagus racemosus root extract via the enhancement of brain-derived neurotrophic factor and estrogen receptor in ovariectomized rats.

Author information

1
Department of Physiology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand.
2
Department of Anatomy, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand.
3
Department of Obstetrics and Gynecology, Faculty of Medicine, Naresuan University, Phitsanulok 65000, Thailand.
4
Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, Thailand.
5
Department of Physiology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand; Center of Excellence in Medical Biotechnology, Naresuan University, Phitsanulok, Thailand. Electronic address: onraweek@nu.ac.th.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Asparagus racemosus (AR) is well known as an Ayurvedic rasayana which used traditionally by Ayurvedic practitioners for nervous disorders and prevent aging. In our previous study it was found that ethanol AR root extract can improve learning and memory impairment, induced by an ovariectomy, but the extract's mechanisms as a neuroprotective property are still unknown.

AIM OF THE STUDY:

This study aimed to examine the effects and mechanisms of ethanol AR root extract on the alteration of brain-derived neurotrophic factor (BDNF) and estrogen receptor (ER) subtypes in ovariectomized (OVX) rats.

MATERIALS AND METHODS:

Adult female Wistar rats were divided into five groups, 4 groups underwent ovariectomy, and one group was designed to be the sham control group. Two groups were gavaged with propylene glycol for sham, and a second group similarly prepared for OVX. Two further groups of OVX rats were gavaged once daily, one group with 100 mg/kg b.w. of ethanol AR root extract and the second group with 1000 mg/kg b.w. of ethanol AR root extract. The fifth group was gavaged once daily with 0.1 mg/kg b.w. of 17α-ethynylestradiol (EE). BDNF, ERα and ERβ expression were evaluated by western blot analysis.

RESULTS:

The western blot analysis revealed that the OVX rats showed a significant decrease in BDNF and a down-regulation of ERα and ERβ in the frontal cortex and hippocampus. It was also demonstrated that EE and AR root extract increased BDNF, ERα and ERβ in the frontal cortex and hippocampus of ovariectomized rats.

CONCLUSIONS:

Based on these results, the enhancement of BDNF and ERs up-regulation may be involved in the neuroprotective effects of ethanol AR root extract in ovariectomized rat.

KEYWORDS:

Asparagus racemosus; Brain-derived neurotrophic factor; Estrogen receptor; Ovariectomy

PMID:
30009979
DOI:
10.1016/j.jep.2018.07.014
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center