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Stand Genomic Sci. 2018 Jul 11;13:16. doi: 10.1186/s40793-018-0318-y. eCollection 2018.

A standardized workflow for submitting data to the Minimum Information about a Biosynthetic Gene cluster (MIBiG) repository: prospects for research-based educational experiences.

Author information

1
1Department of Chemistry, Haverford College, Haverford, PA 19041-1391 USA.
2
2Bioinformatics Group, Wageningen University, Droevendaalsesteeg 1, 6708PB Wageningen, The Netherlands.

Abstract

Microorganisms utilize complex enzymatic pathways to biosynthesize structurally complex and pharmacologically relevant molecules. These pathways are encoded by gene clusters and are found in a diverse set of organisms. The Minimum Information about a Biosynthetic Gene cluster repository facilitates standardized and centralized storage of experimental data on these gene clusters and their molecular products, by utilizing user-submitted data to translate scientific discoveries into a format that can be analyzed computationally. This accelerates the processes of connecting genes to chemical structures, understanding biosynthetic gene clusters in the context of environmental diversity, and performing computer-assisted design of synthetic gene clusters. Here, we present a Standard Operating Procedure, Excel templates, a tutorial video, and a collection of relevant review literature to support scientists in their efforts to submit data into MiBIG. Further, we provide tools to integrate gene cluster annotation projects into the classroom environment, including workflows and assessment materials.

KEYWORDS:

Biosynthetic gene cluster; Course-based undergraduate research experience; MIBiG; Natural product; Specialized metabolism

Conflict of interest statement

Not applicable.Not applicable.Marnix Medema is on the scientific advisory board of Hexagon Bio.

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