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Eur Spine J. 2018 Oct;27(10):2609-2620. doi: 10.1007/s00586-018-5687-9. Epub 2018 Jul 14.

Lupeol against high-glucose-induced apoptosis via enhancing the anti-oxidative stress in rabbit nucleus pulposus cells.

Author information

1
Qingdao Spine Center, Qingdao Municipal Hospital, Qingdao University, Qingdao, 266061, China.
2
Qingdao Spine Center, Qingdao Municipal Hospital, Qingdao University, Qingdao, 266061, China. dechun-w@163.com.

Abstract

PURPOSE:

This study aimed to investigate the potential mechanism and value of lupeol in inhibiting high-glucose-induced apoptosis in rabbit nucleus pulposus cells (NPCs).

METHODS:

NPCs were divided into four groups: control (CON), high glucose (HG), LUP, and HG + LUP. Viability, reactive oxygen species (ROS) levels, and apoptosis were examined in NPCs. The protein expression levels of Bax, Bcl-2, cytochrome C, and caspase 9/3 were measured using reverse transcription-polymerase chain reaction and Western blot assay.

RESULTS:

The apoptotic rate and total ROS level of the HG group significantly increased compared with the CON group (P < 0.01). The total ROS level in the HG + LUP group significantly decreased compared with the HG group(P < 0.05). The mRNA expression of Bcl-2 was significantly upregulated, whereas the expression of Bax, cytochrome C, and caspase 9/3 was downregulated in the HG + LUP group compared with those in the HG group(P < 0.05).The Western blot assay showed that the expression of Bcl-2 was upregulated, but the expression of Bax, cytochrome C, and caspase 9/3 was significantly downregulated in the HG + LUP group compared with the HG group (P < 0.05).

CONCLUSIONS:

Lupeol inhibited high-glucose-induced apoptosis in NPCs by enhancing the anti-oxidative stress in the mitochondria. This study suggested lupeol as a potential therapeutic drug for treating intervertebral disc degeneration under hyperglycaemic conditions. These slides can be retrieved under Electronic Supplementary Material.

KEYWORDS:

Anti-oxidative stress; Apoptosis; Intervertebral disc degeneration; Lupeol; Nucleus pulposus

PMID:
30008063
DOI:
10.1007/s00586-018-5687-9

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