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Cell. 2018 Jul 12;174(2):465-480.e22. doi: 10.1016/j.cell.2018.06.035.

A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced Brain-Cell-Type Targeting and Functionality.

Author information

1
Allen Institute for Brain Science, Seattle, WA 98109, USA.
2
Departments of Neurobiology and Bioengineering, Stanford University School of Medicine, Stanford, CA 94305, USA.
3
MIT Media Lab and McGovern Institute, Departments of Biological Engineering and Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
4
Allen Institute for Brain Science, Seattle, WA 98109, USA. Electronic address: hongkuiz@alleninstitute.org.

Abstract

Modern genetic approaches are powerful in providing access to diverse cell types in the brain and facilitating the study of their function. Here, we report a large set of driver and reporter transgenic mouse lines, including 23 new driver lines targeting a variety of cortical and subcortical cell populations and 26 new reporter lines expressing an array of molecular tools. In particular, we describe the TIGRE2.0 transgenic platform and introduce Cre-dependent reporter lines that enable optical physiology, optogenetics, and sparse labeling of genetically defined cell populations. TIGRE2.0 reporters broke the barrier in transgene expression level of single-copy targeted-insertion transgenesis in a wide range of neuronal types, along with additional advantage of a simplified breeding strategy compared to our first-generation TIGRE lines. These novel transgenic lines greatly expand the repertoire of high-precision genetic tools available to effectively identify, monitor, and manipulate distinct cell types in the mouse brain.

KEYWORDS:

Cre; Flp; TIGRE; calcium sensor; cell type; channelrhodopsin; optogenetics; reporter; transgenic mice; voltage sensor

Comment in

PMID:
30007418
PMCID:
PMC6086366
[Available on 2019-07-12]
DOI:
10.1016/j.cell.2018.06.035
[Indexed for MEDLINE]

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