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Stem Cell Res. 2018 Aug;31:27-30. doi: 10.1016/j.scr.2018.05.007. Epub 2018 May 17.

Generation of integration-free induced pluripotent stem cells from a patient with spina bifida.

Author information

1
Department of Pediatrics, Dell Pediatric Research Institute, Dell Medical School, University of Texas at Austin, Austin, TX 78723, United States.
2
Department of Surgery and Perioperative Care, Dell Medical School, University of Texas at Austin, Austin, TX 78723, United States.
3
Department of Pediatrics, Dell Pediatric Research Institute, Dell Medical School, University of Texas at Austin, Austin, TX 78723, United States; Department of Surgery and Perioperative Care, Dell Medical School, University of Texas at Austin, Austin, TX 78723, United States.
4
Department of Pediatrics, Dell Pediatric Research Institute, Dell Medical School, University of Texas at Austin, Austin, TX 78723, United States. Electronic address: austin.cooney@austin.utexas.edu.

Abstract

A skin biopsy was obtained from a 14-year-old female patient with a history of Myelomeningocele. Dermal fibroblasts were isolated and reprogrammed with Sendai virus (SeV) vectors encoding OCT3/4, SOX2, KLF4, and c-MYC. The generated induced Pluripotent Stem Cell (iPSC) clones NTDi4_09A were free of genomically integrated reprogramming genes, had a stable normal karyotype and expressed pluripotency markers. The iPSCs formed teratomas in mice, which were differentiated towards derivatives of the three germ layers in vivo. This iPSC line offers a useful resource to study a genetic profile of a patient with spina bifida.

PMID:
30007220
DOI:
10.1016/j.scr.2018.05.007
[Indexed for MEDLINE]
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