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Huan Jing Ke Xue. 2018 Aug 8;39(8):3901-3909. doi: 10.13227/j.hjkx.201711234.

[Effects of Long-term Fertilization on Enzyme Activities in Profile of Paddy Soil Profiles].

[Article in Chinese]

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College of Bioscience and Biotechnology, Hunan Agriculture University, Changsha 410128, China.
Key Laboratory of Subtropical Agriculture Ecology, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.
College of Life Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China.
Key Laboratory of Agricultural Environment in the Middle Reaches of the Yangtze River, Ministry of Agriculture, Hunan Academy of Agricultural Sciences, Changsha 410125, China.


The enzyme activity, which is closely related to soil material cycling (mineralization, transformation, etc.), can reflect soil quality and nutrient status. In order to explore the effect of long-term fertilization on the enzyme activity in paddy soil profile (0-40 cm), soils with organic fertilizer and inorganic fertilizer, and non-fertilized soils were selected, and the carbon and nitrogen contents, and the activities of β-1,4-glucosidase (BG), and β-1,4-N-acetylglucosaminidase (NAG) in 10cm depths of soil were analyzed. The results showed that the activities of BG and NAG in the soils treated with inorganic fertilizer and organic fertilizer increased by 0.73-47.87 nmol·(g·h)-1 and 1.33-128.81 nmol·(g·h)-1, and 0.19-9.72 nmol·(g·h)-1 and 0.92-57.66 nmol·(g·h)-1, respectively, compared to those for non-fertilized soil. Soil enzyme activity decreased with increasing soil depth. Soil enzyme activity in soil from 0-20 cm was significantly higher than that of soil from 20-40 cm. Soil enzyme activities were significantly affected by long term fertilization at different soil depths. RDA analysis showed that soil carbon and nitrogen contents had significant positive relationships with the activities of BG and NAG in the 0-20 cm soil profiles, however, negative relationships were observed in the 20-40 cm soil profiles. The long-term application of organic fertilizer significantly increased soil biomass and enzyme activity, both of which decreased with the increase in soil depth. Long-term fertilization could increase soil nutrient contents, microbial biomass, and extracellular enzyme activities, which has important theoretical significance for optimizing farmland fertilizer management and improving soil productivity.


Michaelis-Menten equation; carbon and nitrogen hydrolytic enzyme; enzyme activity; long-term fertilization; paddy soil; soil profile

[Indexed for MEDLINE]

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