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J Cell Mol Med. 2018 Sep;22(9):4377-4386. doi: 10.1111/jcmm.13732. Epub 2018 Jul 11.

Aldolase A as a prognostic factor and mediator of progression via inducing epithelial-mesenchymal transition in gastric cancer.

Author information

1
Department of Gastroenterology, The First People's Hospital of Yancheng, Yancheng, China.
2
Department of Gastroenterology, The Second Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China.
3
Departments of CyberKnife, Huashan Hospital, Fudan University, Shanghai, China.
4
Department of Digestive Endoscopy, The First People's Hospital of Wujiang District, Suzhou, China.

Abstract

Glycolysis is regarded as the hallmark of cancer development and progression, which involves a multistep enzymatic reaction. This study aimed to explore the clinicopathological significance and potential role of glycolytic enzyme aldolase A (ALDOA) in the carcinogenesis and progression of gastric cancer (GC). ALDOA was screened from three paired liver metastasis tissues and primary GC tissues and further explored with clinical samples and in vitro studies. The ALDOA protein level significantly correlated with a larger tumor diameter (P = .004), advanced T stage (P < .001), N stage (P < .001) and lymphovascular invasion (P = .001). Moreover, the expression of ALDOA was an independent prognostic factor for the 5-year overall survival and disease-free survival of patients with GC in both univariate and multivariate survival analyses (P < .05). Silencing the expression of ALDOA in GC cell lines significantly impaired cell growth, proliferation and invasion ability (P < .05). Knockdown of the expression of ALDOA reversed the epithelial-mesenchymal transition process. Mechanically, ALDOA could affect the hypoxia-inducible factor (HIF)-1α activity as demonstrated by the HIF-1α response element-luciferase activity in GC cells. Collectively, this study revealed that ALDOA was a potential biomarker of GC prognosis and was important in the carcinogenesis and progression of human GC.

KEYWORDS:

ALDOA ; epithelial-mesenchymal transition; gastric cancer; survival analysis

PMID:
29992789
PMCID:
PMC6111871
DOI:
10.1111/jcmm.13732
[Indexed for MEDLINE]
Free PMC Article

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