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Plant Biotechnol J. 2019 Feb;17(2):397-409. doi: 10.1111/pbi.12985. Epub 2018 Aug 10.

The red bayberry genome and genetic basis of sex determination.

Author information

1
Institute of Fruit Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
2
Hangzhou 1 Gene Ltd, Hangzhou, China.
3
Forestry Technology Extension Center, Yuyao, Ningbo, China.
4
The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
5
Shanghai Center for Plant Stress Biology, and National Key Laboratory of Plant Molecular Genetics, Center of Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.
6
Institute of Crop Science & Institute of Bioinformatics, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
7
Forest & Fruit Tree Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.
8
Institute of Forestry, Ningbo Academy of Agricultural Science, Ningbo, China.
9
Shunmei Breeding and Propagation Centre for Chinese Bayberry, Yuyao, China.
10
Forestry Technology Extension Center, Cixi, China.
11
Plant Breeding-Wageningen University and Research, Wageningen, The Netherlands.

Abstract

Morella rubra, red bayberry, is an economically important fruit tree in south China. Here, we assembled the first high-quality genome for both a female and a male individual of red bayberry. The genome size was 313-Mb, and 90% sequences were assembled into eight pseudo chromosome molecules, with 32 493 predicted genes. By whole-genome comparison between the female and male and association analysis with sequences of bulked and individual DNA samples from female and male, a 59-Kb region determining female was identified and located on distal end of pseudochromosome 8, which contains abundant transposable element and seven putative genes, four of them are related to sex floral development. This 59-Kb female-specific region was likely to be derived from duplication and rearrangement of paralogous genes and retained non-recombinant in the female-specific region. Sex-specific molecular markers developed from candidate genes co-segregated with sex in a genetically diverse female and male germplasm. We propose sex determination follow the ZW model of female heterogamety. The genome sequence of red bayberry provides a valuable resource for plant sex chromosome evolution and also provides important insights for molecular biology, genetics and modern breeding in Myricaceae family.

KEYWORDS:

Morella rubra; genome; sex-determining region; sex-linked marker

PMID:
29992702
PMCID:
PMC6335074
DOI:
10.1111/pbi.12985
[Indexed for MEDLINE]
Free PMC Article

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