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Clin Exp Allergy. 2018 Oct;48(10):1354-1363. doi: 10.1111/cea.13229. Epub 2018 Aug 14.

Tropomyosins in mosquito and house dust mite cross-react at the humoral and cellular level.

Author information

1
Complutense University of Madrid, Madrid, Spain.
2
Institute for Immunological Research/University of Cartagena, Cartagena, Colombia.
3
Inmunotek S.L., Alcalá de Henares, Spain.
4
University of South Florida College of Medicine, Tampa, Florida.
5
Floridsdorf Allergy Center (FAZ), Vienna, Austria.
6
Allergy Clinic Reumannplatz, Vienna, Austria.
7
Division of Structural Biology, Institute of Molecular Biosciences, BioTechMed University of Graz, Graz, Austria.
8
Department of Pathophysiology and Allergy Research, Medical University of Vienna, Vienna, Austria.

Abstract

BACKGROUND:

Aedes aegypti and Dermatophagoides pteronyssinus contain important allergens including cross-reactive tropomyosins. However, the functional and clinical relevance of their cross-reactivity is still debated.

OBJECTIVE:

To analyse the humoral and cellular cross-reactivity of recombinant Aed a 10.01, Aed a 10.02 and Der p 10.

METHODS:

Sera from 15 Austrian house dust mite-allergic, Der p 10-sensitized individuals were tested for IgE reactivity to recombinant tropomyosins in ELISA, inhibition ELISA and basophil activation tests. BALB/c mice were immunized with Aed a 10.01 or Aed a 10.02, and their sera were assessed for reactivity to all tropomyosins. Splenocytes were stimulated with all tropomyosins and synthetic peptides representing the amino acid sequence of Aed a 10.01.

RESULTS:

IgE antibodies of Der p 10-sensitized patients cross-reacted with both tropomyosins from A. aegypti. Aed a 10.01 was a more potent inhibitor of IgE binding to Der p 10 and a stronger activator of basophils sensitized with Der p 10-specific IgE than Aed a 10.02. Murine antibodies raised against Aed a 10.01 and Aed a 10.02 cross-reacted with Der p 10. Aed a 10.01-specific antibody showed stronger cross-reactivity with Der p 10 than Aed a 10.02-specific antibody. Splenocytes from both groups of mice proliferated similarly to all tropomyosins. Five cross-reactive T cell-activating regions were identified.

CONCLUSION AND CLINICAL RELEVANCE:

Tropomyosins from D. pteronyssinus and A. aegypti show humoral and cellular cross-reactivity, involving 5 potential T cell-activating regions. The more pronounced cross-reactivity of Aed a 10.01 and Der p 10 matched the higher sequence similarity of both proteins.

KEYWORDS:

T-cell epitopes; cross-reactivity; house dust mites; mosquito; tropomyosin

PMID:
29992665
DOI:
10.1111/cea.13229

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