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Biomed Pharmacother. 2018 Oct;106:646-654. doi: 10.1016/j.biopha.2018.06.151. Epub 2018 Jul 11.

Downregulation of miR-218-5p promotes invasion of oral squamous cell carcinoma cells via activation of CD44-ROCK signaling.

Author information

1
Department of Oral Pathology, College of Stomatology, Dalian Medical University, Dalian, China.
2
Institute of Cancer Stem Cell, Dalian Medical University, Dalian, China.
3
Department of Biochemistry and Molecular Biology, Liaoning Provincial Core Lab of Glycobiology and Glycoengineering, Dalian Medical University, Dalian, China.
4
Faculty of Chemical, Environmental and Biological Science and Technology, Dalian Technology University, Dalian, China.
5
Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
6
Department of Oral Pathology, College of Stomatology, Dalian Medical University, Dalian, China. Electronic address: Tingjiao@dlmedu.edu.cn.

Abstract

The invasion front of oral squamous cell carcinoma (OSCC) harbors the most aggressive cells of the tumor and is critical for cancer invasion and metastasis. MicroRNAs (miRNAs) play important roles in OSCC progression. In this study, we modelled the OSCC invasion front on a microfluidic chip, and investigated differences in miRNA profiles between cells in the invasion front and those in the tumor mass by small RNA sequencing. We found that miR-218-5p was downregulated in invasion front cells and negatively regulates OSCC invasiveness by targeting the CD44-ROCK pathway. Thus, miR-218-5p may serve as a useful therapeutic target for OSCC. Moreover, invasion front cell isolation based-on microfluidic technology provided a useful strategy for cancer invasion study.

KEYWORDS:

CD44; Invasion; Microfluidic chip; OSCC; ROCK; miR-218-5p

PMID:
29990854
DOI:
10.1016/j.biopha.2018.06.151
[Indexed for MEDLINE]

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