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Curr Protoc Mouse Biol. 2018 Mar;8(1):17-27. doi: 10.1002/cpmo.38. Epub 2018 Mar 30.

Production of Humanized Mice through Stem Cell Transfer.

Author information

1
Department of Veterinary Biosciences, The Ohio State University, 1925 Coffey Road, Columbus OH 43210, Tel: 614-688-3605.

Abstract

The development of humanized mice has become a prominent tool for translational animal studies of human diseases. Here we show how immune deficient mice can be "humanized" by injections of human umbilical cord stem cells. The engraftment of these cells and development into human lymphocytes has been possible because of the development of novel severely immune deficient mouse strains. Here we present proven protocols for the generation and analysis of humanized mice on the NSG mouse background.

KEYWORDS:

Human Immune System (HIS); Humanized mice; NOD scid gamma (NSG); human umbilical cord stem cells (HUSC)

PMID:
29988984
PMCID:
PMC6034996
DOI:
10.1002/cpmo.38
[Indexed for MEDLINE]
Free PMC Article

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