Format

Send to

Choose Destination
Methods Mol Biol. 2018;1809:45-57. doi: 10.1007/978-1-4939-8570-8_4.

Isolation and Characterization of Mouse Neutrophils.

Author information

1
Department of Medicine, University of Vermont College of Medicine, Burlington, VT, USA.
2
Department of Medicine, University of Vermont College of Medicine, Burlington, VT, USA. benjamin.suratt@uvm.edu.

Abstract

Isolation of murine neutrophils from several anatomical compartments allows for functional characterization and analysis of these cells. Here we describe the isolation of bone marrow, peripheral blood, and lung airspace and interstitial neutrophil populations, using density gradient separation, lavage, and flow cytometry techniques.

KEYWORDS:

Blood; Density gradient; Isolation; Lung; Marrow; Neutrophils

PMID:
29987781
DOI:
10.1007/978-1-4939-8570-8_4
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center