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Methods Mol Biol. 2018;1829:395-406. doi: 10.1007/978-1-4939-8654-5_26.

AT_CHLORO: The First Step When Looking for Information About Subplastidial Localization of Proteins.

Author information

1
Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Commissariat à l'Energie Atomique et aux Energies Alternatives, Grenoble, France.
2
Laboratoire de Biologie à Grande Echelle, Université Grenoble Alpes, Commissariat à l'Energie Atomique et aux Energies Alternatives, Institut National de la Santé et de la Recherche Médicale, Grenoble, France.
3
Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble Alpes, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Commissariat à l'Energie Atomique et aux Energies Alternatives, Grenoble, France. norbert.rolland@cea.fr.

Abstract

Plastids contain several key subcompartments. The two limiting envelope membranes (inner and outer membrane of the plastid envelope with an intermembrane space between), an aqueous phase (stroma), and an internal membrane system terms (thylakoids) formed of flat compressed vesicles (grana) and more light structures (lamellae). The thylakoid vesicles delimit another discrete soluble compartment, the thylakoid lumen. AT_CHLORO ( http://at-chloro.prabi.fr/at_chloro/ ) is a unique database supplying information about the subplastidial localization of proteins. It was created from simultaneous proteomic analyses targeted to the main subcompartments of the chloroplast from Arabidopsis thaliana (i.e., envelope, stroma, thylakoid) and to the two subdomains of thylakoid membranes (i.e., grana and stroma lamellae). AT_CHLORO assembles several complementary information (MS-based experimental data, curated functional annotations and subplastidial localization, links to other public databases and references) which give a comprehensive overview of the current knowledge about the subplastidial localization and the function of chloroplast proteins, with a specific attention given to chloroplast envelope proteins.

KEYWORDS:

Chloroplast; Envelope; Plant; Proteomics; Stroma; Subcellular localization; Thylakoid

PMID:
29987736
DOI:
10.1007/978-1-4939-8654-5_26
[Indexed for MEDLINE]

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