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Mult Scler. 2019 Oct;25(11):1535-1538. doi: 10.1177/1352458518786055. Epub 2018 Jul 9.

Cerebrospinal fluid mitochondrial DNA levels in patients with multiple sclerosis.

Author information

1
Servei de Neurologia-Neuroimmunologia, Centre d'Esclerosi Múltiple de Catalunya (Cemcat), Institut de Recerca Vall d'Hebron (VHIR), Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.
2
Memory Unit, Department of Neurology, IIB Sant Pau, Hospital de la Santa Creu i Sant Pau, Universitat Autònoma de Barcelona, Barcelona, Spain.
3
Department of Immunology, Hospital Ramón y Cajal, Madrid, Spain.
4
Department of Neurology, Clinic of Neurology, Clinical Center of Serbia, University of Belgrade School of Medicine, Belgrade, Serbia.
5
Department of Neurology, Clinic of Neurology, Clinical Center of Serbia, University of Belgrade School of Medicine, Belgrade, Serbia/ Department of Neurology, UNC School of Medicine, Chapel Hill, NC, USA.
6
Department of Neurology, UNC School of Medicine, Chapel Hill, NC, USA.
7
Department of Neurology, Medical University of Graz, Graz, Austria.

Abstract

The role of cerebrospinal fluid (CSF) mitochondrial DNA (mtDNA) levels as biomarker in multiple sclerosis (MS) is unknown. We determined CSF mtDNA levels in a cohort of 237 individuals, including patients with MS and clinically isolated syndrome (CIS), inflammatory and non-inflammatory neurological controls, and cognitively healthy controls (HC). mtDNA concentration was measured by droplet digital polymerase chain reaction. CSF mtDNA levels were increased in all pathological conditions compared with HC, though no differences were observed between relapse-onset and progressive MS clinical forms, CIS patients and neurological controls. These findings do not support the determination of CSF mtDNA levels as a useful biomarker in MS clinical practice.

KEYWORDS:

Mitochondrial DNA; cerebrospinal fluid; droplet digital PCR; multiple sclerosis

PMID:
29985092
DOI:
10.1177/1352458518786055

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