Advancements in electrochemical DNA sensor for detection of human papilloma virus - A review

Elisa Rasouli; Zohreh Shahnavaz; Wan Jefrey Basirun; Majid Rezayi; Amir Avan; Majid Ghayour-Mobarhan; Roshanak Khandanlou; Mohd Rafie Johan

doi:10.1016/j.ab.2018.07.002

Advancements in electrochemical DNA sensor for detection of human papilloma virus - A review

Anal Biochem. 2018 Sep 1:556:136-144. doi: 10.1016/j.ab.2018.07.002. Epub 2018 Jul 5.

Authors

Elisa Rasouli¹, Zohreh Shahnavaz², Wan Jefrey Basirun³, Majid Rezayi⁴, Amir Avan⁵, Majid Ghayour-Mobarhan⁶, Roshanak Khandanlou⁷, Mohd Rafie Johan⁸

Affiliations

¹ Nanotechnology & Catalysis Research Centre, Institute of Postgraduate Studies, University of Malaya, 50603, Kuala Lumpur, Malaysia; Department of Chemistry, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia. Electronic address: elisa.rasouli@gmail.com.
² Nanotechnology & Catalysis Research Centre, Institute of Postgraduate Studies, University of Malaya, 50603, Kuala Lumpur, Malaysia. Electronic address: zohreh.shahnavaz@um.edu.my.
³ Nanotechnology & Catalysis Research Centre, Institute of Postgraduate Studies, University of Malaya, 50603, Kuala Lumpur, Malaysia; Department of Chemistry, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia. Electronic address: jeff@um.edu.my.
⁴ Department of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: chem_rezayi@yahoo.com.
⁵ Department of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: avana@mums.ac.ir.
⁶ Department of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: ghayourm@mums.ac.ir.
⁷ School of Applied and Biomedical Sciences, Faculty of Science and Technology, Federation University, 3350, Ballarat, Australia. Electronic address: roshanak_bch@yahoo.com.
⁸ Nanotechnology & Catalysis Research Centre, Institute of Postgraduate Studies, University of Malaya, 50603, Kuala Lumpur, Malaysia. Electronic address: mrafiej@um.edu.my.

PMID: 29981317
DOI: 10.1016/j.ab.2018.07.002

Abstract

Human papillomavirus (HPV) is one of the most common sexually transmitted disease, transmitted through intimate skin contact or mucosal membrane. The HPV virus consists of a double-stranded circular DNA and the role of HPV virus in cervical cancer has been studied extensively. Thus it is critical to develop rapid identification method for early detection of the virus. A portable biosensing device could give rapid and reliable results for the identification and quantitative determination of the virus. The fabrication of electrochemical biosensors is one of the current techniques utilized to achieve this aim. In such electrochemical biosensors, a single-strand DNA is immobilized onto an electrically conducting surface and the changes in electrical parameters due to the hybridization on the electrode surface are measured. This review covers the recent developments in electrochemical DNA biosensors for the detection of HPV virus. Due to the several advantages of electrochemical DNA biosensors, their applications have witnessed an increased interest and research focus nowadays.

Keywords: Cervical cancer; DNA electrochemical biosensor; Human papilloma virus; Single strand DNA.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Biosensing Techniques / methods*
DNA, Single-Stranded / analysis*
DNA, Viral / analysis*
Electrochemical Techniques / methods*
Humans
Limit of Detection
Papillomaviridae*
Papillomavirus Infections / diagnosis

Substances

DNA, Single-Stranded
DNA, Viral