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Biotechnol Bioeng. 2018 Oct;115(10):2467-2478. doi: 10.1002/bit.26790. Epub 2018 Aug 11.

Techniques for chromosomal integration and expression optimization in Escherichia coli.

Ou B1,2,3,4, Garcia C4, Wang Y5, Zhang W4, Zhu G1,2,3.

Author information

1
College of Veterinary Medicine, Yangzhou University, Yangzhou, China.
2
Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and Zoonoses, Yangzhou, China.
3
Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, China.
4
Diagnostic Medicine/Pathobiology, Kansas State University College of Veterinary Medicine, Manhattan, Kansas.
5
Department of Cell Biology and Genetics, School of Basic Medical Sciences, Shenzhen University Health Science Center, Shenzhen, China.

Abstract

Due to the inherent expression stability and low metabolic burden to the host cell, the expression of heterologous proteins in the bacterial chromosome in a precise and efficient manner is highly desirable for metabolic engineering and live bacterial applications. However, obtaining suitable chromosome expression levels is particularly challenging. In this minireview, we briefly present the technologies available for the integration of heterologous genes into Escherichia coli chromosomes and strategies to optimize the expression levels of heterologous proteins.

KEYWORDS:

Escherichia coli (E. coli); chromosomal expression; chromosomal integration; expression cassette design; optimization; synthetic biology

PMID:
29981268
DOI:
10.1002/bit.26790

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