Adoptive Transfer of Fluorescently Labeled Immune Cells in Xenopus

Kun Hyoe Rhoo; Jacques Robert

doi:10.1101/pdb.prot097592

Adoptive Transfer of Fluorescently Labeled Immune Cells in Xenopus

Cold Spring Harb Protoc. 2019 May 1;2019(5):10.1101/pdb.prot097592. doi: 10.1101/pdb.prot097592.

Authors

Kun Hyoe Rhoo¹, Jacques Robert²

Affiliations

¹ Department of Immunology Microbiology and Virology, University of Rochester, Medical Center, New York 14620 Kunhyoe_rhoo@urmc.rochester.edu.
² Department of Immunology Microbiology and Virology, University of Rochester, Medical Center, New York 14620.

Abstract

Adoptive cell transfer from inbred adult Xenopus to inbred tadpoles is a useful way to study the dissemination of immune cells or pathogen-infected immune cells in tadpoles. For example, Xenopus peritoneal leukocytes (PLs) can be readily infected by pathogens such as Frog virus 3 (FV3) and Mycobacterium marinum (M. marinum). By transferring fluorescently labeled, FV3-infected PLs into tadpoles, we observed infiltration of these cells into the tadpole's brain, which indicates that FV3-infected PLs can cross blood brain barrier. Taking advantage of tadpoles' transparency, fluorescently labeled immune cells can be tracked in real time using fluorescence microscopy.

MeSH terms

Adoptive Transfer / methods*
Animals
Fluorescent Dyes / metabolism*
Leukocytes / metabolism*
Microscopy, Fluorescence
Staining and Labeling*
Xenopus laevis / immunology*

Substances

Fluorescent Dyes

Abstract

MeSH terms

Substances

Grants and funding