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PLoS One. 2018 Jul 6;13(7):e0200280. doi: 10.1371/journal.pone.0200280. eCollection 2018.

HappyTools: A software for high-throughput HPLC data processing and quantitation.

Author information

1
Research & Development, Ludger Limited., Abingdon, Oxfordshire, United Kingdom.
2
Department of Rheumatology, Leiden University Medical Center, Leiden, Zuid-Holland, The Netherlands.
3
Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, Zuid-Holland, The Netherlands.

Abstract

High-performance liquid chromatography (HPLC) is widely used for absolute quantitation. The advent of new columns and HPLC technology has enabled higher sample throughput, and hence, larger scale studies that perform quantitation on different sample types (e.g. healthy controls vs. patients with rheumatoid arthritis) using HPLC are becoming feasible. However, there remains a lack of methods that can analyse the increased number of HPLC samples. To address this in part, the modular toolkit HappyTools has been developed for the high-throughput targeted quantitation of HPLC measurements. HappyTools enables the user to create an automated workflow that includes retention time (tr) calibration, data extraction and the calculation of several quality criteria for data curation. HappyTools has been tested on a biopharmaceutical standard and previously published clinical samples. The results show comparable accuracy between HappyTools, Waters Empower and ThermoFisher Chromeleon. However, HappyTools offered superior precision and throughput when compared with Waters Empower and ThermoFisher Chromeleon. HappyTools is released under the Apache 2.0 license, both the source code and a Windows binary can be freely downloaded from https://github.com/Tarskin/HappyTools.

PMID:
29979768
PMCID:
PMC6034860
DOI:
10.1371/journal.pone.0200280
[Indexed for MEDLINE]
Free PMC Article

Conflict of interest statement

The authors have declared that no competing interests exist. The software published in this article has been partially developed at Ludger. However, the software is released completely open source which is why the authors have declared no competing interests. BCJ, RAG, JLH and DIRS work for Ludger Ltd – a commercial company that specialises in the development and validation of glycoprofiling technology for biologic therapeutics and biological tissues. Some of the Ludger products include glycan purification kits and glycan release kits. There are no patents and products in development. This does not alter the authors’ adherence to PLOS ONE policies on sharing data and materials.

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