Send to

Choose Destination
PLoS One. 2018 Jul 6;13(7):e0200275. doi: 10.1371/journal.pone.0200275. eCollection 2018.

Yeast lifespan variation correlates with cell growth and SIR2 expression.

Author information

Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX, United States of America.
Center for the Integration of STEM Education & Research, Texas Tech University, Lubbock, TX, United States of America.
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, United States of America.
Department of Medical Education, Texas Tech University Health Sciences Center, Lubbock, TX, United States of America.


Isogenic wild type yeast cells raised in controlled environments display a significant range of lifespan variation. Recent microfluidic studies suggest that differential growth or gene expression patterns may explain some of the heterogeneity of aging assays. Herein, we sought to complement this work by similarly examining a large set of replicative lifespan data from traditional plate assays. In so doing, we reproduced the finding that short-lived cells tend to arrest at senescence with a budded morphology. Further, we found that wild type cells born unusually small did not have an extended lifespan. However, large birth size and/or high inter-generational growth rates significantly correlated with a reduced lifespan. Finally, we found that SIR2 expression levels correlated with lifespan and intergenerational growth. SIR2 expression was significantly reduced in large cells and increased in small wild type cells. A moderate increase in SIR2 expression correlated with reduced growth, decreased proliferation and increased lifespan in plate aging assays. We conclude that cellular growth rates and SIR2 expression levels may contribute to lifespan variation in individual cells.

[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Public Library of Science Icon for PubMed Central
Loading ...
Support Center