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J Proteome Res. 2018 Dec 7;17(12):4325-4328. doi: 10.1021/acs.jproteome.8b00384. Epub 2018 Jul 6.

Identification of the Missing Protein Hyaluronan Synthase 1 in Human Mesenchymal Stem Cells Derived from Adipose Tissue or Umbilical Cord.

Author information

1
Proteomics Unit, Faculty of Pharmacy , Complutense University of Madrid (UCM) , Plaza Ramón y Cajal s/n , 28040 Madrid , Spain.
2
Proteobotics SL , Darwin 3 , 28049 Madrid , Spain.
3
Andalusian Public Health System Biobank , Avenida Del Conocimiento s/n , 18016 Granada , Spain.
4
Department of Microbiology & Parasitology, Faculty of Pharmacy , Complutense University of Madrid (UCM) and Ramón y Cajal Institute of Health Research (IRYCIS) , Plaza Ramón y Cajal s/n , 28040 Madrid , Spain.
5
Proteomics Unit , Spanish National Biotechnology Centre (CNB-CISC) , Darwin 3 , 28049 Madrid , Spain.

Abstract

Currently, 14% of the human proteome is made up of proteins whose existence is not confirmed by mass spectrometry. We performed a proteomic profiling of human mesenchymal stem cells derived from adipose tissue or umbilical cord (PRIDE accession number: PXD009893) and identified peptides derived from 13 of such missing proteins. Remarkably, we found compelling evidence of the expression of hyaluronan synthase 1 (NX_Q92839-1) and confirmed its identification by the fragmentation of four heavy-labeled peptides that coeluted with their endogenous light counterparts. Our data also suggest that mesenchymal stem cells constitute a promising source for the detection of missing proteins.

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