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Open Biol. 2018 Jul;8(7). pii: 180029. doi: 10.1098/rsob.180029.

Cultured cells and wing disc size of silkworm can be controlled by the Hippo pathway.

Liang Z1,2, Lu Y1,2, Qian Y1,2, Zhu L1,2, Kuang S1,2, Chen F1,2, Feng Y1,2, Hu X1,3, Cao G1,3, Xue R4,3, Gong C5,3,2.

Author information

1
School of Biology and Basic Medical Science, Agricultural Biotechnology and Ecological Research Institute, Soochow University, Suzhou, 215123, China.
2
Agricultural Biotechnology Research Institute, Agricultural Biotechnology and Ecological Research Institute, Soochow University, Suzhou, 215123, China.
3
National Engineering Laboratory for Modern Silk, Agricultural Biotechnology and Ecological Research Institute, Soochow University, Suzhou, 215123, China.
4
School of Biology and Basic Medical Science, Agricultural Biotechnology and Ecological Research Institute, Soochow University, Suzhou, 215123, China xuery@suda.edu.cn.
5
School of Biology and Basic Medical Science, Agricultural Biotechnology and Ecological Research Institute, Soochow University, Suzhou, 215123, China gongcl@suda.edu.cn.

Abstract

Hippo signalling represents a cell proliferation and organ-size control pathway. Yorki (Yki), a component of the Hippo pathway, induces the transcription of a number of targets that promote cell proliferation and survival. The functions of Yki have been characterized in Drosophila and mammals, while there are few reports on silkworm, Bombyx mori In the present study, we found that BmYki3 facilitates cell migration and cell division, and enlarges the cultured cell and wing disc size. Co-immunoprecipitation results indicated that BmYki3 may interact with thymosin, E3 ubiquitin-protein ligase, protein kinase ASK1, dedicator of cytokinesis protein 1, calcium-independent phospholipase A2 and beta-spectrin. RNA-seq results indicated that 4444 genes were upregulated and 10 291 genes were downregulated after BmYki3 was overexpressed in the cultured cells. GO annotation indicated that the up/downregulated genes were enriched in 268/382 GO terms (p < 0.01); KEGG analysis showed that the up/downregulated genes were enriched in 49/101 pathways. These findings provided novel information to understand the functions of BmYki3 in a cell proliferation and organ-size control pathway.

KEYWORDS:

Bombyx mori; cell proliferation; differential gene expression; wing disc size; yorki

PMID:
29973396
PMCID:
PMC6070717
DOI:
10.1098/rsob.180029
[Indexed for MEDLINE]
Free PMC Article

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