Format

Send to

Choose Destination
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2018 Apr;34(4):359-363.

[Resveratrol promotes ATP production and the proliferation of human granulosa cells in vitro].

[Article in Chinese]

Author information

1
Navy Clinical College, Anhui Medical University, Beijing 100048; Department of Obstetrics and Gynecology, PLA Navy General Hospital, Beijing 100048, China.
2
Department of Obstetrics and Gynecology, Chinese PLA General Hospital, Chinese PLA Medical School, Beijing 100853, China.
3
Navy Clinical College, Anhui Medical University, Beijing 100048; Department of Obstetrics and Gynecology, PLA Navy General Hospital, Beijing 100048, China. *Corresponding author, E-mail: 3313400243@qq.com.

Abstract

Objective To explore the effects of resveratrol (Res) on the proliferation and ATP production of human ovarian granulosa cells in vitro. Methods Human follicular granulosa cells obtained from the follicle fluid of infertile patients were purified and identified by immunocytochemistry for follicle stimulating hormone receptor (FSHR), and then cultured with medium in vitro. The cells were divided into a blank control group, a solvent control group (DMSO 30 μmol/L), and Res-treated group (10, 20 and 30 μmol/L). Cell viability was detected by CCK-8 assay. Cellular ATP levels were measured using the ATP assay kit after 24 hours in Res-treated group (10 μmol/L) and control group. Results FSHR was immunoreactive-positive in cytoplasm of 90% isolated cells. The proliferation of granulosa cells reached the peak during 24 to 72 hours. Compared with the control group, the proliferation activity of the human follicular granulosa cells treated with Res (10, 20 and 30 μmol/L) for 24 hours significantly increased. The level of ATP in the Res-treated group (10 μmol/L) increased compared with the control group after treatment with Res for 24 hours. Conclusion Resveratrol promotes the proliferation activity and increases the ATP production of human granulosa cells in vitro.

PMID:
29973328
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center