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Nanomedicine (Lond). 2018 Jun;13(12):1417-1432. doi: 10.2217/nnm-2018-0011. Epub 2018 Jul 4.

1α,25-dihydroxyvitamin D3-eluting nanofibrous dressings induce endogenous antimicrobial peptide expression.

Author information

1
Department of Surgery, Transplant & Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE 68198, USA.
2
Department of Biochemistry & Biophysics, Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA.
3
Nutrition Graduate Program, School of Biological & Population Health Sciences, College of Public Health & Human Sciences, Oregon State University, Corvallis, OR 97331, USA.
4
Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR 97331, USA.
5
Department of Dermatology, Oregon Health & Science University (OHSU), Portland, OR 97239, USA.
6
Knight Cancer Institute, OHSU, Portland, OR 97239, USA.
7
Department of Surgery - Plastic & Reconstructive Surgery, University of Nebraska Medical Center, Omaha, NE 68198, USA.
8
Department of Surgery - General Surgery & Genetics, Cell Biology & Anatomy, University of Nebraska Medical Center, Omaha, NE 68198, USA.
9
Department of Surgery, VA Nebraska - Western Iowa Health Care System, Omaha, NE 68105, USA.
10
Department of Dermatology, University of California, San Diego, CA 92093, USA.

Abstract

AIM:

The aim of this study was to develop a nanofiber-based dressing capable of local sustained delivery of 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) and augmenting human CAMP induction.

MATERIALS & METHODS:

Nanofibrous wound dressings containing 1,25(OH)2D3 were successfully prepared by electrospinning, which were examined in vitro, in vivo and ex vivo.

RESULTS:

1,25(OH)2D3 was successfully loaded into nanofibers with encapsulation efficiency larger than 90%. 1,25(OH)2D3 showed a sustained release from nanofibers over 4 weeks. Treatment of U937 and HaCaT cells with 1,25(OH)2D3-loaded poly(ϵ-caprolactone) nanofibers significantly induced hCAP18/LL37 expression in monocytes and keratinocytes, skin wounds of humanized transgenic mice and artificial wounds of human skin explants.

CONCLUSION:

1,25(OH)2D3 containing nanofibrous dressings could enhance innate immunity by inducing antimicrobial peptide production.

KEYWORDS:

1α,25-dihydroxyvitamin D3; antimicrobial peptides LL-37; electrospun nanofibers; endogenous production; innate immunity; sustained delivery

PMID:
29972648
PMCID:
PMC6219435
[Available on 2019-06-01]
DOI:
10.2217/nnm-2018-0011
[Indexed for MEDLINE]

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