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Elife. 2018 Jul 4;7. pii: e34025. doi: 10.7554/eLife.34025.

Multiple inputs ensure yeast cell size homeostasis during cell cycle progression.

Garmendia-Torres C1,2,3,4, Tassy O1,2,3,4, Matifas A1,2,3,4, Molina N1,2,3,4, Charvin G1,2,3,4.

Author information

1
Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France.
2
Centre National de la Recherche Scientifique, UMR7104, Illkirch, France.
3
Institut National de la Santé et de la Recherche Médicale, U1258, Illkirch, France.
4
Université de Strasbourg, Illkirch, France.

Abstract

Coordination of cell growth with division is essential for proper cell function. In budding yeast, although some molecular mechanisms responsible for cell size control during G1 have been elucidated, the mechanism by which cell size homeostasis is established remains to be discovered. Here, we developed a new technique based on quantification of histone levels to monitor cell cycle progression in individual cells with unprecedented accuracy. Our analysis establishes the existence of a mechanism controlling bud size in G2/M that prevents premature onset of anaphase, and controls the overall size variability. While most G1 mutants do not display impaired size homeostasis, mutants in which cyclin B-Cdk regulation is altered display large size variability. Our study thus demonstrates that size homeostasis is not controlled by a G1-specific mechanism alone but is likely to be an emergent property resulting from the integration of several mechanisms that coordinate cell and bud growth with division.

KEYWORDS:

S. cerevisiae; cell biology; cell cycle; computational biology; microfluidics; size control; systems biology

PMID:
29972352
PMCID:
PMC6085122
DOI:
10.7554/eLife.34025
[Indexed for MEDLINE]
Free PMC Article

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