Format

Send to

Choose Destination
J Exp Clin Cancer Res. 2018 Jul 3;37(1):134. doi: 10.1186/s13046-018-0803-6.

Long non-coding RNA UFC1 promotes gastric cancer progression by regulating miR-498/Lin28b.

Zhang X1,2, Liang W3,4, Liu J5, Zang X3,4, Gu J5, Pan L3,4, Shi H3,4, Fu M3,4, Huang Z3,4, Zhang Y3, Qian H3,4, Jiang P4, Xu W6,7.

Author information

1
Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu, China. xuzhang@ujs.edu.cn.
2
Institute of Digestive Diseases of Jiangsu University, The Affiliated People's Hospital of Jiangsu University, 8 Dianli Road, Zhenjiang, 212002, Jiangsu, China. xuzhang@ujs.edu.cn.
3
Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu, China.
4
Institute of Digestive Diseases of Jiangsu University, The Affiliated People's Hospital of Jiangsu University, 8 Dianli Road, Zhenjiang, 212002, Jiangsu, China.
5
Tumor Institute, Nantong Tumor Hospital, 30 Tongyang North Road, Nantong, 226361, Jiangsu, China.
6
Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu, China. icls@ujs.edu.cn.
7
Institute of Digestive Diseases of Jiangsu University, The Affiliated People's Hospital of Jiangsu University, 8 Dianli Road, Zhenjiang, 212002, Jiangsu, China. icls@ujs.edu.cn.

Abstract

BACKGROUND:

Long non-coding RNAs (lncRNAs) have emerged as important regulators of human cancers. However, the functional roles of lncRNAs and the mechanisms responsible for their aberrant expression in gastric cancer (GC) have not been well characterized.

METHODS:

In this study, we examined the expression of lncRNA UFC1 in GC by qRT-PCR and explored its correlation with clinicopathological parameters. In vitro cell functional assays and in vivo animal studies were performed to determine the roles of UFC1 in GC progression.

RESULTS:

UFC1 was elevated and predicted poorer prognosis in GC. UFC1 knockdown inhibited while UFC1 overexpression promoted GC cell proliferation, migration, and invasion. UFC1 bound to miR-498 to antagonize its tumor suppressive effect on Lin28b. Suppression of Lin28b by miR-498 could be rescued by UFC1 overexpression, whereas Lin28b overexpression partially rescued UFC1 knockdown-mediated inhibition of GC cell function. Lin28b expression was increased in GC and suggested a co-expression pattern with UFC1.

CONCLUSIONS:

UFC1 has a promoting role in GC progression, at least in part, by acting as a miR-498 sponge and derepressing Lin28b expression, which would provide a novel biomarker for GC diagnosis and prognosis and offer a potential target for GC therapy.

KEYWORDS:

Biomarker; Gastric cancer; Lin28b; Progression; UFC1; miR-498

PMID:
29970131
PMCID:
PMC6029056
DOI:
10.1186/s13046-018-0803-6
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center