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Bioconjug Chem. 2018 Aug 15;29(8):2734-2740. doi: 10.1021/acs.bioconjchem.8b00385. Epub 2018 Jul 18.

Dendrimer Scaffold for the Amplification of In Vivo Pretargeting Ligations.

Cook BE1,2,3, Membreno R1,2, Zeglis BM1,2,3,4.

Author information

1
Department of Chemistry , Hunter College of the City University of New York , New York , New York 10065 , United States.
2
Ph.D. Program in Chemistry , The Graduate Center of the City University of New York , New York , New York 10016 , United States.
3
Department of Radiology , Memorial Sloan Kettering Cancer Center , New York , New York 10065 , United States.
4
Department of Radiology , Weill Cornell Medical College , New York , New York 10065 , United States.

Abstract

The development of immunoconjugates requires a careful balance between preserving the functionality of the antibody and modifying the immunoglobulin with the desired cargo. Herein, we describe the synthesis, development, and in vivo evaluation of a novel bifunctional dendrimeric scaffold and its application in pretargeted PET imaging. The site-specific modification of the huA33 antibody with this dendrimeric scaffold yields an immunoconjugate-sshuA33-DEN-TCO-decorated with ∼8 trans-cyclooctene (TCO) moieties, a marked increase compared to the ∼2 TCO/mAb of a nondendrimeric control immunoconjugate (sshuA33-PEG12-TCO). Pretargeted PET imaging and biodistribution experiments were used to compare the in vivo performance of these two immunoconjugates in athymic nude mice bearing subcutaneous SW1222 human colorectal cancer xenografts. To this end, the mice were administered 100 μg of each immunoconjugate followed 120 h later by the injection of a tetrazine-bearing radioligand, [64Cu]Cu-SarAr-Tz. Pretargeting with sshuA33-DEN-TCO produced excellent tumoral uptake at 24 h (8.9 ± 1.9 %ID/g), more than double that created by sshuA33-PEG12-TCO (4.1 ± 1.3 %ID/g). Critically-and somewhat surprisingly-the attachment of the G0.5 dendrimeric structures did not hamper the in vivo behavior of the immunoconjugate, suggesting that this versatile bifunctional scaffold may have applications beyond pretargeting.

PMID:
29969558
PMCID:
PMC6432625
DOI:
10.1021/acs.bioconjchem.8b00385
[Indexed for MEDLINE]
Free PMC Article

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