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Phytopathology. 2018 Dec;108(12):1455-1466. doi: 10.1094/PHYTO-04-18-0120-R. Epub 2018 Nov 9.

Novel Insights Into the Early Stages of Ratoon Stunting Disease of Sugarcane Inferred from Transcript and Protein Analysis.

Author information

1
First, second, third, fourth, eighth, ninth, and eleventh authors: Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Av. Pádua Dias, 11, 13418-900, Piracicaba, SP, Brazil; fifth and seventh authors: Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, 05508-900, São Paulo, SP, Brazil; sixth author: Instituto Butantan, Laboratório Especial de Toxinologia Aplicada, Av. Vital Brasil, 1500, 05503-900, São Paulo, SP, Brazil; and tenth author: Department of Plant Sciences, University of California, Davis 95616.

Abstract

Despite of the importance of ratoon stunting disease, little is known on the responses of sugarcane to its causal agent, the vascular bacterial endophyte Leifsonia xyli subsp. xyli. The transcriptome and proteome of young plants of a susceptible cultivar with no symptoms of stunting but with relative low and high bacterial titers were compared at 30 and 60 days after inoculation. Increased bacterial titers were associated with alterations in the expression of 267 cDNAs and in the abundance of 150 proteins involved in plant growth, hormone metabolism, signal transduction and defense responses. Some alterations are predicted to benefit the pathogen, such as the up-regulation of genes involved in the synthesis of methionine. Also, genes and proteins of the cell division cycle were all down-regulated in plants with higher titers at both times. It is hypothesized that the negative effects on cell division related to increased bacterial titers is cumulative over time and its modulation by other host and environmental factors results in the stunting symptom.

KEYWORDS:

microarray; plant−pathogen interaction

PMID:
29969065
DOI:
10.1094/PHYTO-04-18-0120-R
[Indexed for MEDLINE]

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