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Sci Rep. 2018 Jul 3;8(1):9990. doi: 10.1038/s41598-018-28245-9.

Actin polymerization is activated by terahertz irradiation.

Author information

1
Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University, Aramaki Aza Aoba 468-1, Aoba-ku, Sendai, 980-0845, Japan. shota.yamazaki.fc@riken.jp.
2
Terahertz Sensing and Imaging Research Team, RIKEN Center for Advanced Photonics, 519-1399 Aramaki-Aoba, Aoba-ku, Sendai, Miyagi, 980-0845, Japan. shota.yamazaki.fc@riken.jp.
3
Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University, Aramaki Aza Aoba 468-1, Aoba-ku, Sendai, 980-0845, Japan. masahiko.harata.b6@tohoku.ac.jp.
4
Research Center for Development of Far-Infrared Region, University of Fukui (FIR UF), Bunkyo 3-9-1, Fukui, 910-8507, Japan.
5
Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto, 606-8205, Japan.
6
Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University, Aramaki Aza Aoba 468-1, Aoba-ku, Sendai, 980-0845, Japan.
7
Terahertz Sensing and Imaging Research Team, RIKEN Center for Advanced Photonics, 519-1399 Aramaki-Aoba, Aoba-ku, Sendai, Miyagi, 980-0845, Japan.
8
Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto, 606-8205, Japan. ogawayu@kais.kyoto-u.ac.jp.

Abstract

Polymerization of monomeric actin into filaments has pivotal roles in cell motility, growth, differentiation, and gene expression. Therefore, techniques of manipulating actin polymerization, including actin-binding chemicals, have been developed for understanding and regulating multiple biological functions. Here, we demonstrate that irradiation with terahertz (THz) waves is a novel method of modulating actin polymerization. When actin polymerization reaction is performed under irradiation with 0.46 THz waves generated by a Gyrotron, actin polymerization was observed to be activated by monitoring the fluorescence of pyrene actin fluorophores. We also observed the number of actin filaments under a fluorescence microscope using the polymerized actin probe SiR-actin. The number of actin filaments was increased by 3.5-fold after THz irradiation for 20 min. When the THz irradiation was applied to a steady-state actin solution, in which elongation and depolymerization of actin filaments were equilibrated, increased actin polymerization was observed, suggesting that the THz irradiation activates actin polymerization, at least in the elongation process. These results suggest that THz waves could be applied for manipulating biomolecules and cells.

PMID:
29968762
PMCID:
PMC6030223
DOI:
10.1038/s41598-018-28245-9
[Indexed for MEDLINE]
Free PMC Article

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