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Nat Methods. 2018 Jul;15(7):519-522. doi: 10.1038/s41592-018-0042-y. Epub 2018 Jul 2.

Chemogenetic control of gene expression and cell signaling with antiviral drugs.

Author information

1
Department of Biomedical Engineering and Biological Design Center, Boston University, Boston, MA, USA.
2
Department of Biomedical Engineering and Biological Design Center, Boston University, Boston, MA, USA. jtngo@bu.edu.

Abstract

We developed a method in which the NS3 cis-protease from hepatitis C virus can be used as a ligand-inducible connection to control the function and localization of engineered proteins in mammalian cells. To demonstrate the versatility of this approach, we designed drug-sensitive transcription factors and transmembrane signaling proteins, the activities of which can be tightly and reversibly controlled through the use of clinically tested antiviral protease inhibitors.

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PMID:
29967495
DOI:
10.1038/s41592-018-0042-y
[Indexed for MEDLINE]

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