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Huan Jing Ke Xue. 2018 May 8;39(5):2400-2411. doi: 10.13227/j.hjkx.201708085.

[Diversity of the Microbial Community in Rice Paddy Soil with Biogas Slurry Irrigation Analyzed by Illumina Sequencing Technology].

[Article in Chinese]

Author information

1
Research Center for Environmental Monitoring, Hazard Prevention of Three Gorges Reservoir, Yangtze Normal University, Chongqing 408100, China.
2
Collaborative Innovation Center for Green Development in Wuling Mountain Areas, Yangtze Normal University, Chongqing 408100, China.
3
Chongqing Academy of Agricultural Sciences, Chongqing 401329, China.
4
School of Life Science, Chongqing Normal University, Chongqing 401331, China.
5
College of Resources and Environment, Southwest University, Chongqing 400715, China.

Abstract

In order to explore the variation in soil microbial community diversity in paddy fields with different irrigation periods, we collected in situ rice field soils during different biogas irrigation periods and analyzed the microbial community structures of these soils by high-throughput sequencing. The results showed that as the biogas irrigation period increased, the soil pH decreased gradually, while organic matter, nitrate nitrogen, phosphate, and other nutrients were accumulated. Years of continued biogas irrigation was not conducive to improving rice yields. The results showed that as the biogas irrigation period increased, the richness in microbial species in paddy soils decreased gradually, and the diversity in the microbial communities was also reduced. Proteobacteria accounts for the largest proportion in rice paddy soil with biogas slurry irrigation. With the increase of biogas irrigation years, the proportion of β-Proteobacteria, Bacteroidia, Bacteroidales, Burkholderiales, Bacteroides, and Thiobacillus increased, while the proportion of Gemmatimonadetes and α-Proteobacteria decreased gradually. Dissolved organic carbon (F=2.67, P=0.09) had the greatest effect on microbial community structures in the studied paddy soils.

KEYWORDS:

Illumina sequencing technology; biogas slurry; diversity; microorganism; rice paddy

PMID:
29965541
DOI:
10.13227/j.hjkx.201708085
[Indexed for MEDLINE]

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