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Mol Biochem Parasitol. 2018 Jul;223:31-36. doi: 10.1016/j.molbiopara.2018.06.004. Epub 2018 Jun 28.

Revealing genetic hybridization and DNA recombination of Fasciola hepatica and Fasciola gigantica in nuclear introns of the hybrid Fasciola flukes.

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Walai Rukhavej Botanical Research Institute (WRBRI), Biodiversity and Conservation Research Unit, Mahasarakham University, Maha Sarakham, 44150, Thailand.
Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Rangsit Campus, Pathumthani, 12120, Thailand. Electronic address:
Department of Environmental Medicine, Kochi Medical School, Kochi University, Oko, Nankoku, 783-8505, Japan.
The First Affiliated Hospital of Jiamusi University, Jiamusi University, Jiamusi, Heilongjiang Province, People's Republic of China.
Department of Parasitology, Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, 40002, Thailand.
Institute of Zoology 1: Ecology and Parasitology, University of Karlsruhe, Kornblumen Strasse 13, Karlsruhe, Germany; State Museum of Natural History, Erbprinzenstr. 13, Karlsruhe, 76133, Germany.


Fasciola hepatica and F. gigantica are ruminant liver flukes that are found worldwide. They can occur sympatrically and hybridize, consequently producing an intermediate form (Fasciola sp.), especially in Africa and Asia. The bridge intron (TkBridgeInt) and intron 4 of domain 2 (TkD2Int4) of the taurocyamine kinase gene were cloned and sequenced from 14 F. hepatica, 18 F. gigantica, and 12 Fasciola sp. These intron regions could be used to differentiate F. hepatica from F. gigantica and revealed genetic hybridization and DNA recombination between F. hepatica and F. gigantica in Fasciola sp. from Japan and Bangladesh. Two specimens of F. hepatica from Ecuador revealed cryptic diversity. A combination of three haplotypes observed in a Fasciola sp. specimen from Japan suggested triploidy. Thus, the intron region of the taurocyamine kinase gene is a potential marker for detecting the hybrid status of the Fasciola flukes.


Fascioliasis; Fasciolidae; Haplotype network; Heterozygous; Taurocyamine kinase

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