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World J Gastroenterol. 2018 Jun 28;24(24):2596-2604. doi: 10.3748/wjg.v24.i24.2596.

Novel serum microRNAs panel on the diagnostic and prognostic implications of hepatocellular carcinoma.

Author information

1
Department of Hepato-Biliary-Pancreatic Surgey, Navy General Hospital of Chinese People's Liberation Army, Beijing 100048, China.
2
Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Interventional Therapy Department, Peking University Cancer Hospital & Institute, Beijing 100142, China.
3
The Liver Disease Center for Cadre Medical Care, Beijing 302 Military Hospital, Beijing 100039, China. fanzp302@126.com.

Abstract

AIM:

To determine a panel of serum microRNAs (miRNAs) that could be used as novel biomarkers for diagnosis of hepatocellular carcinoma (HCC).

METHODS:

We initially screened 9 out of 754 serum miRNAs by TaqMan Low Density Array in two pooled samples respectively from 35 HCC and 35 normal controls, and then validated individually by RT-qPCR in another 114 patients and 114 controls arranged in two phases. The changes of the selected miRNAs after operation and their prognostic value were examined.

RESULTS:

miR-375, miR-10a, miR-122 and miR-423 were found to be significantly higher in HCC than in controls (P < 0.0001), and the area under the receiver-operating-characteristic curve for the 4-miRNA panel was 0.995 (95%CI: 0.985-1). All the four miRNAs were significantly reduced after surgical removal of the tumors (P < 0.0001), while still higher than normal controls (at least P < 0.05).

CONCLUSION:

The four serum miRNAs (miR-375, miR-10a, miR-122 and miR-423) could potentially serve as novel biomarkers for the diagnostic and prognostic of HCC.

KEYWORDS:

Biomarker; Diagnosis; Hepatocellular carcinoma; MicroRNAs; Serum

PMID:
29962816
PMCID:
PMC6021775
DOI:
10.3748/wjg.v24.i24.2596
[Indexed for MEDLINE]
Free PMC Article

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