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Biochim Biophys Acta Mol Basis Dis. 2018 Sep;1864(9 Pt B):3085-3098. doi: 10.1016/j.bbadis.2018.06.022. Epub 2018 Jun 28.

TIGIT-Fc alleviates acute graft-versus-host disease by suppressing CTL activation via promoting the generation of immunoregulatory dendritic cells.

Author information

1
Department of Plastic Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China; Transplant Immunology Laboratory, School of Basic Medical Sciences, Fourth Military Medical University, Xi'an 710032, China.
2
Transplant Immunology Laboratory, School of Basic Medical Sciences, Fourth Military Medical University, Xi'an 710032, China; Department of Immunology, School of Basic Medical Sciences, Fourth Military Medical University, Xi'an 710032, China.
3
Transplant Immunology Laboratory, School of Basic Medical Sciences, Fourth Military Medical University, Xi'an 710032, China; Department of Emergency, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
4
Transplant Immunology Laboratory, School of Basic Medical Sciences, Fourth Military Medical University, Xi'an 710032, China.
5
Department of Immunology, School of Basic Medical Sciences, Fourth Military Medical University, Xi'an 710032, China.
6
Department of Plastic Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
7
Transplant Immunology Laboratory, School of Basic Medical Sciences, Fourth Military Medical University, Xi'an 710032, China; Department of Immunology, School of Basic Medical Sciences, Fourth Military Medical University, Xi'an 710032, China. Electronic address: fmmuzhr@fmmu.edu.cn.

Abstract

Graft-versus-host disease (GVHD) is the most common complication and major limitation of allogeneic hematopoietic stem cell transplantation. The CD226/TIGIT-CD155 signal is critical for the cross-talk between T cells and dendritic cells (DCs). Studies have shown that blockade of the CD226-CD155 interaction, using an anti-CD226 antibody, can significantly ameliorate GVHD. It has also been reported that a TIGIT-Fc fusion protein exerts immunosuppressive effects by binding to CD155 on DCs. Here, we used a mouse allogeneic acute GVHD model to explore the therapeutic potential and mechanism of action of TIGIT-Fc. C57/BL6 and Balb/c mice were used as hematopoietic cell graft donors and recipients, respectively. In the TIGIT-Fc-treated mice, GVHD symptom occurrence and mortality were delayed compared to that in isotype control group mice. Histopathological analyses revealed that following TIGIT-Fc treatment, liver and small intestine tissue damage was reduced with minimal lymphocytic infiltration. The percentage of CD8+IFN-γ+ and CD8+ granzyme B+ cells significantly decreased in the TIGIT-Fc group. Moreover, treatment with TIGIT-Fc, even after the onset of GVHD, ameliorated symptoms and prolonged survival. TIGIT-Fc also inhibited CD8+ T cell activation in vitro; this was dependent on the presence of CD155 on bone marrow-derived dendritic cells (BMDCs) and on IL-10 production. In addition, TIGIT-CD155 ligation triggered both Erk phosphorylation and STAT3 nuclear translocation. These data indicate that TIGIT plays an important role in the development of GVHD and is an ideal molecular target to treat acute GVHD.

KEYWORDS:

CD155; CD8(+) T cells; Dendritic cells; Graft-versus-host disease; TIGIT

PMID:
29960041
DOI:
10.1016/j.bbadis.2018.06.022
[Indexed for MEDLINE]
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