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Epigenomics. 2018 Jul;10(7):1011-1026. doi: 10.2217/epi-2017-0178. Epub 2018 Jun 29.

DNA methylation of imprinted genes in Mexican-American newborn children with prenatal phthalate exposure.

Author information

1
Center for Environmental Research & Children's Health (CERCH), School of Public Health, University of California, Berkeley, CA 94720, USA.
2
Epigenetics Research Laboratory, Department of Obstetrics & Gynecology, Duke University Medical Center, Durham, NC 27708, USA.
3
Department of Biological Sciences, Center for Human Health & the Environment, North Carolina State University (NCSU), Raleigh, NC 27606, USA.

Abstract

AIM:

Imprinted genes exhibit expression in a parent-of-origin-dependent manner and are critical for child development. Recent limited evidence suggests that prenatal exposure to phthalates, ubiquitous endocrine disruptors, can affect their epigenetic dysregulation.

MATERIALS & METHODS:

We quantified DNA methylation of nine imprinted gene differentially methylated regions by pyrosequencing in 296 cord blood DNA samples in a Mexican-American cohort. Fetal exposure was estimated by phthalate metabolite concentrations in maternal urine samples during pregnancy.

RESULTS:

Several differentially methylated regions of imprinted genes were associated with high molecular weight phthalates. The most consistent, positive, and false discovery rate significant associations were observed for MEG3.

CONCLUSION:

Phthalate exposure in utero may affect methylation status of imprinted genes in newborn children.

KEYWORDS:

DNA methylation; Mexican–Americans; cord blood; differentially methylated region; endocrine disruptors; imprinted genes; in utero exposure; newborns; phthalates; pyrosequencing

PMID:
29957030
PMCID:
PMC6088267
DOI:
10.2217/epi-2017-0178
[Indexed for MEDLINE]
Free PMC Article

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