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Development. 2018 Jul 23;145(14). pii: dev164848. doi: 10.1242/dev.164848.

Induced cell fate transitions at multiple cell layers configure haustorium development in parasitic plants.

Author information

1
Graduate School of Science, The University of Tokyo, Bunkyo, Tokyo 113-0033, Japan.
2
RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan.
3
Institute for Research Initiatives, Division for Research Strategy, Nara Institute of Science and Technology, Ikoma, Nara 630-0192, Japan.
4
Graduate School of Biological Science, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan.
5
Graduate School of Science, The University of Tokyo, Bunkyo, Tokyo 113-0033, Japan ken.shirasu@riken.jp.

Abstract

The haustorium in parasitic plants is an organ specialized for invasion and nutrient uptake from host plant tissues. Despite its importance, the developmental processes of haustoria are mostly unknown. To understand the dynamics of cell fate change and cellular lineage during haustorium development, we performed live imaging-based marker expression analysis and cell-lineage tracing during haustorium formation in the model facultative root parasite Phtheirospermum japonicum Our live-imaging analysis revealed that haustorium formation was associated with induction of simultaneous cell division in multiple cellular layers, such as epidermis, cortex and endodermis. In addition, we found that procambium-like cells, monitored by cell type-specific markers, emerged within the central region of the haustorium before xylem connection to the host plant. Our clonal analysis of cell lineages showed that cells in multiple cellular layers differentiated into procambium-like cells, whereas epidermal cells eventually transitioned into specialized cells interfacing with the host plant. Thus, our data provide a cell fate transition map during de novo haustorium organogenesis in parasitic plants.

KEYWORDS:

CASP1; CESA7; Cell fate transition; Cellular reprogramming; HB15; Haustorium formation; Organogenesis; Parasitic plant; WOX4

PMID:
29950390
PMCID:
PMC6078332
DOI:
10.1242/dev.164848
[Indexed for MEDLINE]
Free PMC Article

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