Format

Send to

Choose Destination
Chem Commun (Camb). 2018 Jul 10;54(56):7806-7809. doi: 10.1039/c8cc03285h.

Charge designable and tunable GFP as a target pH-responsive carrier for intracellular functional protein delivery and tracing.

Author information

1
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China. huangyan.hnu@gmail.com niezhou.hnu@gmail.com.

Abstract

A charge designable and tunable green fluorescent protein (GFP)-based protein delivery strategy was proposed. The acquired His29GFP selectively permeates the cell membrane at a target pH of 6.5 and escapes from the endosome efficiently. The delivered RNase A caused substantial mRNA degradation in HeLa cells, and proliferation inhibition in different cell lines and a 3D tumor model at pH 6.5.

PMID:
29946615
DOI:
10.1039/c8cc03285h

Supplemental Content

Full text links

Icon for Royal Society of Chemistry
Loading ...
Support Center