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Dev Comp Immunol. 2018 Oct;87:188-192. doi: 10.1016/j.dci.2018.06.002. Epub 2018 Jun 23.

Increased antiviral capacity of transgenic silkworm via knockdown of multiple genes on Bombyx mori bidensovirus.

Author information

1
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China.
2
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China. Electronic address: jiangliang@swu.edu.cn.
3
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, PR China. Electronic address: zhaop@swu.edu.cn.

Abstract

Bombyx mori bidensovirus (BmBDV) causes fatal flacherie disease leading to severe economic losses in sericultures. The BmDNV-Z genome contains two single-stranded DNA molecules, VD1 and VD2. For generating silkworm lines with antiviral properties, two transgenic RNA interference (RNAi) vectors were constructed. Open reading frames (ORFs) 1-4 of VD1 were knockdown by vector pb-BDV1 while ORF1a, ORF1b, and ORF3 of VD2 were knockdown by vector pb-BDV2. Transgenic silkworm lines BDV1-I and BDV2-I were generated via RNAi microinjection. Mortality rates of BDV1-I and BDV2-I were reduced by 45% and 39%, respectively, and quantitative PCR showed that VD1 and VD2 contents in BDV1-I and BDV2-I were significantly lower than in the non-transgenic line. However, economic traits showed no obvious differences. Thus, knockdown of multiple BmDNV-Z genes provides strong resistance to BDV1-I and BDV2-I lines, and these can be used in sericulture without hampering silk production.

KEYWORDS:

Antiviral capacity; Bombyx mori bidensovirus; RNAi; Silkworm; Transgenic

PMID:
29944898
DOI:
10.1016/j.dci.2018.06.002
[Indexed for MEDLINE]

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