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Curr Protoc Mouse Biol. 2018 Sep;8(3):e48. doi: 10.1002/cpmo.48. Epub 2018 Jun 26.

A Method for Parasagittal Sectioning for Neuroanatomical Quantification of Brain Structures in the Adult Mouse.

Collins SC1,2,3,4,5, Wagner C1,2,3,4, Gagliardi L1,2,3,4, Kretz PF1,2,3,4, Fischer MC1,2,3,4, Kessler P1,2,3,4, Kannan M1,2,3,4, Yalcin B1,2,3,4.

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Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France.
Centre National de la Recherche Scientifique, UMR7104, Illkirch, France.
Institut National de la Santé et de la Recherche Médicale, U964, Illkirch, France.
Université de Strasbourg, Illkirch, France.
Centre des Sciences du Goût et de l'Alimentation, Université de Bourgogne-Franche Comté, Dijon, France.


In this article, we present a standardized protocol for fast and robust neuroanatomical phenotyping of the adult mouse brain, which complements a previously published article (doi: 10.1002/cpmo.12) in Current Protocols in Mouse Biology. It is aimed at providing an experimental pipeline within an academic research setting from experimental work to data analysis. Our analysis focuses on one single parasagittal plane, covering the majority of brain regions involved in higher order cognitions such as the cortex, hippocampus, and cerebellum, for a total of 166 parameters of area, length, and cell-level measurements in contrast to 78 parameters in our previously published coronal screen. Benefits of using parasagittal analysis for large-scale neuroanatomic screens are discussed.


brain morphogenesis; histology; mouse phenotyping; parasagittal sectioning; ultra-standardized procedure

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