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Food Chem Toxicol. 2018 Aug;118:880-888. doi: 10.1016/j.fct.2018.06.039. Epub 2018 Jun 20.

Oxyresveratrol stimulates mucin production in an NAD+-dependent manner in human intestinal goblet cells.

Author information

1
Department of Biomedical Laboratory Science, College of Life and Health Sciences, Hoseo University, Asan, 31499, Republic of Korea.
2
Department of Integrated Biomedical and Life Sciences, Graduate School, Korea University, Seoul, 02841, Republic of Korea.
3
Department of Integrated Biomedical and Life Sciences, Graduate School, Korea University, Seoul, 02841, Republic of Korea; Department of Public Health Science (BK21 PLUS Program), Graduate School, Korea University, Seoul, 02841, Republic of Korea; Department of Laboratory Medicine, Korea University Guro Hospital, Seoul, 08308, Republic of Korea. Electronic address: yhlim@korea.ac.kr.

Abstract

The intestinal mucus layer plays an important role in the management of inflammatory bowel disease. The aim of this study was to investigate the effects of oxyresveratrol (OXY), an antioxidant, on the stimulation of mucin production in human LS 174T goblet cells and the underlying mechanism thereof. OXY increased MUC2 expression at both the mRNA and protein levels. By performing two-dimensional gel electrophoresis, we found that the expression of nicotinic acid phosphoribosyltransferase1 (NaPRT1) in OXY-treated LS 174T cells was greatly increased compared with that in negative control cells. In addition, the NAD+/NADH ratio was increased in proportion to OXY in LS 174T cells. The expression of NAD+-synthesis enzymes, NaPRT1, nicotinamide riboside kinase1 (NRK1) and nicotinamide mononucleotide adenylyltransferase1 (Nmnat1) was significantly increased at both the mRNA and protein levels in OXY-treated LS 174T cells. The inhibition of NaPRT1 and NRK1 did not decrease MUC2 expression after inhibiting by small interfering RNA (siRNA)-NaPRT1 and siRNA-NRK1, respectively; however, inhibition of Nmnat by an Nmnat inhibitor decreased MUC2 expression in a dose-dependent manner. In conclusion, OXY increases NAD+ levels, resulting in the stimulation of MUC2 expression in LS 174T cells. These findings present a novel role for NAD+ in stimulation of MUC2 expression.

KEYWORDS:

Goblet cells; Intestinal mucus layer; MUC2; NAD(+); Oxyresveratrol

PMID:
29935245
DOI:
10.1016/j.fct.2018.06.039
[Indexed for MEDLINE]

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